Signalling

Part:BBa_K1909001:Design

Designed by: Daniel Cortés   Group: iGEM16_Hamburg   (2016-10-13)


pathogen associated molecular pattern sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 171
    Illegal SapI.rc site found at 523


Design Notes

To prevent premature ribosome drop-off, the pair of CTA codons (L77 and L79 of the LRR domain) predicted to strongly stall the translation was substituted by TTA codons. An XbaI restriction site was removed by changing L239 from CTA to TTA. To make up for the lost translational pause, L242 was changed from CTG to CTA (Fig 1C). We also added an eight aminoacid FLAG-tag at residue 16, allowing for expression confirmation by targeted purification.



Source

EnvZ - http://www.uniprot.org/uniprot/P0AEJ4 NOD1 - http://www.uniprot.org/uniprot/Q9Y239

References